Organoids bridge a gap in existing model systems by providing a more stable system amenable to extended cultivation and manipulation while being more representative of in vivo physiology. Three-dimensional ex vivo organoid cultures using biomaterial-based assembly and self-assembly have been shown to resemble and recapitulate most of the functionality of diverse multicellular tissues and organs, such as the gut organoids. Moreover, the discovery of substrates, pathways, and growth factors involved in the differentiation of organoids and stem cells into specific lineages is expected to contribute significantly to the clinical protocols. Our team explored ex vivo gut organoid models to study new oral vaccine delivery systems by determining how artificial virus-like nanoparticle-based (AVN) vaccine transport within an ex vivo intestinal mucosal immunological system built by primary intestinal stem cells derived gut organoids (Miniguts). Our research showed that the mammalian orthoreovirus cell attachment protein σ1 (MRV σ1) functionalized AVNs resulted in targeted vaccine delivery through induced M-cells in the intestinal epithelium.
“Organoid as a New Platform to Develop Oral Vaccines and Nanomedicines”
Qun Wang, PhD
Assistant Professor, Department of Chemical and Biological Engineering, Iowa State University
Thursday, October 28th, 3:00–4:00 PM CT
If you have any questions about this event, please contact Carly Ross, firstname.lastname@example.org, 515-294-2194